People

Group leader

Dr. Stefan Düsterhöft

Curriculum vitae (English): CV_eng.pdf

sduesterhoeft@ukaachen.de

Selected Publications:

[1] Kahveci-Türköz, S., K. Bläsius, J. Wozniak, C. Rinkens, A. Seifert, P. Kasparek, H. Ohm, S. Oltzen, M. Nieszporek, N. Schwarz, A. Babendreyer, C. Preisinger, R. Sedlacek, A. Ludwig and S. Düsterhöft (2023) “A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity.” Cell Mol Life Sci DOI: 10.1007/s00018-023-04783-y.

[2] Niehues, R.V., J. Wozniak, E. Lilienthal, F. Wiersch, N. Tacken, T. Schumertl, C. Garbers, A. Ludwig and S. Düsterhöft (2022). "The collectrin-like part of the SARS-CoV-1 and -2 receptor ACE2 is shed by the metalloproteinases ADAM10 and ADAM17” FASEB J 36:e22234. DOI: 10.1096/fj.202101521R

[3] Düsterhöft, S., S. Kahveci-Turkoz, J. Wozniak, A. Seifert, P. Kasparek, H. Ohm, S. Liu, J. Kopkanova, J. Lokau, C. Garbers, C. Preisinger, R. Sedlacek, M. Freeman and A. Ludwig (2021). "The iRhom homology domain is indispensable for ADAM17-mediated TNFalpha and EGF receptor ligand release." Cell Mol Life Sci 78(11):5015-5040. DOI: 10.1007/s00018-021-03845-3

[4] Düsterhöft, S., A. K. Bartels, T. Koudelka, E. Lilienthal, M. Schäfer, C. Garbers, A. Tholey, J. Grötzinger and I. Lorenzen (2020). "Distance dependent shedding of IL-6R." Biochem Biophys Res Commun 526(2): 355-360. DOI: 10.1016/j.bbrc.2020.03.093

[5] Lorenzen, I., J. Lokau, Y. Korpys, M. Oldefest, C. M. Flynn, U. Künzel, C. Garbers, M. Freeman, J. Grötzinger and S. Düsterhöft (2016). "Control of ADAM17 activity by regulation of its cellular localisation." Sci Rep 6: 35067. DOI: 10.1038/srep35067

[6] Düsterhöft, S., M. Michalek, F. Kordowski, M. Oldefest, A. Sommer, J. Röseler, K. Reiss, J. Grötzinger and I. Lorenzen (2015). "Extracellular Juxtamembrane Segment of ADAM17 Interacts with Membranes and Is Essential for Its Shedding Activity." Biochemistry 54(38): 5791-5801. DOI: 10.1021/acs.biochem.5b00497

[7] Düsterhöft, S., K. Hobel, M. Oldefest, J. Lokau, G. H. Waetzig, A. Chalaris, C. Garbers, J. Scheller, S. Rose-John, I. Lorenzen and J. Grötzinger (2014). "A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor." J Biol Chem 289(23): 16336-16348. DOI: 10.1074/jbc.M114.557322

[8] Düsterhöft, S., S. Jung, C. W. Hung, A. Tholey, F. D. Sönnichsen, J. Grötzinger and I. Lorenzen (2013). "Membrane-proximal domain of a disintegrin and metalloprotease-17 represents the putative molecular switch of its shedding activity operated by protein-disulfide isomerase." J Am Chem Soc 135(15): 5776-5781. DOI: 10.1021/ja400340u

Reviews

[1] Düsterhöft, S., A. Babendreyer, A. A. Giese, C. Flasshove and A. Ludwig (2019). "Status update on iRhom and ADAM17: It's still complicated." Biochim Biophys Acta Mol Cell Res 1866(10): 1567-1583. DOI: 10.1016/j.bbamcr.2019.06.017

[2] Düsterhöft, S., J. Lokau and C. Garbers (2019). "The metalloprotease ADAM17 in inflammation and cancer." Pathol Res Pract 215(6): 152410. DOI: 10.1016/j.prp.2019.04.002

[3] Grötzinger, J., I. Lorenzen and S. Düsterhöft (2017). "Molecular insights into the multilayered regulation of ADAM17: The role of the extracellular region." Biochim Biophys Acta Mol Cell Res 1864(11 Pt B): 2088-2095. DOI: 10.1016/j.bbamcr.2017.05.024

[4] Düsterhöft, S., U. Künzel and M. Freeman (2017). "Rhomboid proteases in human disease: Mechanisms and future prospects." Biochim Biophys Acta Mol Cell Res 1864(11 Pt B): 2200-2209. DOI: 10.1016/j.bbamcr.2017.04.016

 
 

Associated Postdoc

Dr. Justyna Wozniak

Master of Mice and Wizard of Statistics

Contact: jwozniak@ukaachen.de

 

Current Lab Members

 

Selcan (PhD student)

Analysing the iRhom2-ADAM17 Axis in Lung Inflammations

 

Katharina (PhD student)

Novel Interaction Partners of iRhom Pseudoproteases

 

Diandra (Master student)

Novel Interaction Partners of iRhom Pseudoproteases

 

Florian (med. PhD student)

Functional Consequences of ACE2 Shedding

 

Rabea (med. PhD student)

Regulation of ADAM-mediated Shedding of ACE2

 

Maria (med. PhD student)

Regulation of ADAM-mediated Shedding of ACE2

 

Lena (med. PhD student)

Function of the iRhom N-terminus

 

Daniel (Master student)

CRISPR/Cas9

 

Former Lab Members

 

Henrike (Master, 2018)

“Characterization of a highly conserved region within the homology domain of the pseudoproteases iRhom1 and iRhom2”

 

Pascal (Master, 2019)

"Characterisation of the binding between iRhom and ADAM17"

 

Sarah (Bachelor, 2019)

“In vitro characterisation of the N-terminus of the pseudoprotease iRhom2”

 

Shixin (Master, 2020)

“The influence of a highly conserved sequence of Pseudoprotease iRhom1 and iRhom2 on ADAM17”

 

Cindy (Master, 2020)

“Characterisation of the binding between ADAM17 and the pseudoprotease iRhom2 by reactivating the catalytic site”

 

Magdalena (Bachelor, 2020)

"Analysis of the direct effect of a small molecule drug on ADAM17- and ADAM10-mediated shedding"

 

Katharina (Master, 2021)

“Novel interaction partners of iRhom pseudoproteases”

 

Martin (Master, 2021)

“Characterization of the iRhom2/ADAM17 complex localization in iRhom2 mutant cells”

 

Friederike (med. PhD, 2021)

“Characterization of a highly conserved region within the homology domain of the pseudoproteases iRhom1 and iRhom2”

 

Anita (Master student; Research internship, 2022)

The influence of syntaxins on the forward trafficking of the ADAM17-iRhom complex

 

Nikola (Master, 2022)

Analysing N-terminal deletions in iRhom2 constructs in iRhom knock-out cells

 

Eva (technician)

Analysing the Molecular Shedding Mechanism / Identifying New Substrates

 

Xintong (Master, 2022)

Cub and TOC mutations in iRhoms

 

Johanna (Master, 2022)

Regulation of Collectrin and ACE shedding

 

Laura (Bachelor, 2022)

Novel Interaction Partners of iRhom Pseudoproteases

 

Christine (Master, 2023)

Determinants of ACE2 Shedding